-Detailed Information [JCRB0833]-

JCRB0833 NH-12

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0833	Cell Name	NH-12
Profile	neuroblastoma, adrenal gland	Other Name	neuroblastoma-12
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age	1year-3months
Identity	available	Tissue for Primary Cancer	neural
Case history	neuroblastoma	Metastasis
Tissue Metastasized		Genetics
Life Span	infinite	Crisis PDL
Morphology	fibroblast-like	Character	neroblastoma cell line
Classify	tumor	Established by	Hiyama,E. & Nakamura,N.
Registered by	Akiyama,M.	Regulation for Distribution
Comment		Year	1992
Medium	MEM-alpha with 10% fetal calf serum.	Methods for Passages	Cells are treated with 0.25% trypsin and 0.02% EDTA and dilute in fresh medium.
Cell Number on Passage		Race
CO2 Conc.	5 %	Tissue Sampling	adrenal gland
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_1605
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	908447

Reference
Pubmed id:1555997	Length of telomeric repeats in neuroblastoma: correlation with prognosis and other biological characteristics. Hiyama E,Hiyama K,Yokoyama T,Ichikawa T,Matsuura Y Jpn J Cancer Res. 1992 Feb;83(2):159-64

Images

Movies

LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.

073197
041097
090595
090695
10242018

Cell No.	JCRB0833	Cell Name	NH-12
LOT No.	073197	Lot Specification	distribution
Medium	Alpha MEM with 10% fetal calf serum (JRH bio, lot.4B2013).	Temperature	37 C
Cell Density at Seeding	4 x 10^4 cells/sq.cm.	Methods for Passages	Harvest cells with 0.25% trypsin and 0.02% EDTA for 5 minutes at room temperature.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.8x10^6
Viability at cell freezing (%)	99.0	Antibiotics Used	free
Passage Number	P54	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Culture medium with 5% DMSO.	CO2 Conc.
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0833	Cell Name	NH-12
LOT No.	041097	Lot Specification	distribution
Medium	******************************	Temperature
Cell Density at Seeding		Methods for Passages
Doubling Time		Cell Number in Vial (cells/1ml)
Viability at cell freezing (%)		Antibiotics Used
Passage Number		PDL
Sterility: MYCOPLASMA	+	Sterility: BACTERIA
Sterility: FUNGI		Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:10,11 D13S317:8,9 D7S820:11,13 D16S539:11 VWA:17,18 TH01:9,10 AM:XY TPOX:8 CSF1PO:11,12
Adhesion		Exoteric Gene
Medium for Freezing	******************************	CO2 Conc.
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0833	Cell Name
LOT No.	090595	Lot Specification	distribution
Medium	alpha-MEM + FCS 10%	Temperature	37 C
Cell Density at Seeding		Methods for Passages	0.02% EDTA + 0.25 % trypsin
Doubling Time		Cell Number in Vial (cells/1ml)
Viability at cell freezing (%)		Antibiotics Used
Passage Number		PDL
Sterility: MYCOPLASMA		Sterility: BACTERIA
Sterility: FUNGI		Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0833	Cell Name
LOT No.	090695	Lot Specification	distribution
Medium	alpha-MEM + FCS 10%	Temperature	37 C
Cell Density at Seeding		Methods for Passages	0.02% EDTA + 0.25 % trypsin
Doubling Time		Cell Number in Vial (cells/1ml)
Viability at cell freezing (%)		Antibiotics Used
Passage Number		PDL
Sterility: MYCOPLASMA		Sterility: BACTERIA
Sterility: FUNGI		Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0833	Cell Name	NH-12
LOT No.	10242018	Lot Specification	distribution
Medium	Alpha-MEM with 10% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	0.9 - 2.1 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 48 hrs.	Cell Number in Vial (cells/1ml)	3.1 x 10^6
Viability at cell freezing (%)	90.4	Antibiotics Used	free
Passage Number	P58	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:10,11 D13S317:8,9 D7S820:11,13 D16S539:11 VWA:17,18 TH01:9,10 AM:X,Y TPOX:8 CSF1PO:11,12
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - alpha-MEM	CO2 Conc.	5%
Viability immediately after thawing (%)	78.8	Additional information