JCRB1040 FF101
細胞情報
細胞種類:一般細胞 (細胞分譲手数料はこちら)
| 細胞番号(JCRB) | JCRB1040 | 細胞名 | FF101 |
|---|---|---|---|
| 生物種(日本語) | ラット | 組織名(日本語) | 肝臓 |
| コメント(日本語) | 肝細胞がん, 3'-methyl-4-dimethylaminoazobenzene誘発, 無血清培養, (Donryu rat) | プロフィール | Rat hepatoma cell line with producing unidentified growth factor maintained in serum-free RPMI1640 medium for 444 passages. |
| 別名 | 動物名 | rat | |
| 系統名 | donryu | 学名・属名 | Rattus |
| 学名・種小名 | norvegicus | 性別 | |
| 年齢・月齢 | adult | 細胞識別情報 | |
| (癌)原発組織名 | liver | 病歴情報 | hepatoma was induced by feeding 0.06% 3'-methyl-4-dimethylaminoazobenzene for five months. |
| 転移の有無(Y/N) | (癌)転移組織名 | ||
| 遺伝的性質 | Cells have characteristics of hepatoma and cholangioma. | 細胞寿命 | infinite |
| クライシスPDL | 形態 | epithelial-like | |
| 一般性状 | The cells are expected to produce FF-GF (unidentified growth factor, 70KDa). Sup. of the cell culture have growth activating activity(AH66, K562, Y3, YAC-1,BALB/c,3T3,NS-1). Cells have been cultured with serum-free medium from the primary cuture. | 細胞分類 | tumor |
| 細胞樹立者名 | Matsumoto,H.et.al. | 細胞寄託者 | Matsumoto,H |
| 分譲時制限 | not defined | コメント | Cells have been maintained by continuous cell culture without freezing at all. |
| 入手年 | 2002 | 培養培地 | Serum-free, protein-free RPMI1640 with increased concentration of glutamine (double). |
| 継代方法 | Adhesion to dishes a weak. Pipetting with 2-3 times is enough to harvest cells. Subculture once every 7-10 days. | 継代時細胞数 | not defined. |
| 人種 | 炭酸ガス濃度 | 5 % | |
| 採取組織名 | liver | 組織型 |
| ウイルスDNA・RNA検出検査 (Detection of virus genome fragment by Real-time PCR) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| ウイルスDNA 検出検査 |
not tested | ウイルスRNA 検出検査 |
not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:1281996 | Purification and characterization of a novel growth factor (FF-GF) synthesized by a rat hepatoma cell line, FF101. Matsuda H,Matsumoto M,Kitahara H,Haraguchi S,Kanai K Biochem Biophys Res Commun. 1992 Dec 15;189(2):654-61 |
| Pubmed id:2702652 | Partial purification of a growth factor synthesized by a rat hepatoma cell line established in serum-free medium. Matsuda H,Matsumoto M,Haraguchi S,Kanai K Cancer Res. 1989 Apr 15;49(8):2118-22 |
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LOT Information
生細胞率、増殖速度、細胞濃度はバンクでのロット作製時の実測値として記していますが、保証値ではございません。また、倍加時間は継代間で計測したラフな値です。Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
| 細胞番号 | JCRB1040 | 細胞名 | FF101 |
|---|---|---|---|
| 培養ロット番号 | 10282003 | 培養種別 | distribution |
| 培地 | Serum-free, protein-free RPMI1640 with increased concentration of glutamine (double). | 培養温度 | 37C |
| 継代時細胞数(濃度) | 3.4 x 10^4 cells/sq.cm | 継代方法 | Pipetting with 2-3 times is enough to harvest cells. |
| 増殖速度 | NT | 凍結時生細胞濃度 | 1.7 x 10^6 |
| 凍結時生細胞率 | 86.2 | 使用抗生物質 | Free |
| 継代数 | P455 | PDL数(プライマリ) | NT |
| マイコプラズマ検出 | - | 細菌汚染検出 | - |
| 真菌汚染検出 | - | アイソザイム検査・動物名 | NT |
| 染色体モード | NT | 染色体情報 | NT |
| 表面抗原 | NT | DNA Profile (STR) | |
| 接着性 | Yes | 導入外部遺伝子 | NT |
| 凍結培地 | Culture medium + FM-1 (1 to 1 mix) | 炭酸ガス濃度 | 5% |
| 解凍後生細胞率 | 追加情報 |
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