JCRB0169 Lu-134-B
細胞情報
Important Notice(s)Culture medium for JCRB0169 Lu-134-B
細胞種類：一般細胞 (細胞分譲手数料はこちら)
| 細胞番号(JCRB) | JCRB0169 | 細胞名 | Lu-134-B |
|---|---|---|---|
| 生物種(日本語) | ヒト | 組織名(日本語) | 肺(がん) |
| コメント(日本語) | 小細胞がん, classic type | プロフィール | Small cell lung carcinoma cell line established through heterotransplanted tumor. |
| 別名 | 動物名 | human | |
| 系統名 | 学名・属名 | Homo | |
| 学名・種小名 | sapiens | 性別 | M |
| 年齢・月齢 | 80 | 細胞識別情報 | available |
| （癌）原発組織名 | lung (cancer) | 病歴情報 | small cell carcinoma arising in the periphery of right lower lobe of lung. |
| 転移の有無（Y/N） | （癌）転移組織名 | ||
| 遺伝的性質 | Activities of gamma-enolase and aromatic L-amino acid decarboxylase are high significantly. Hypertetraploid chromosome pattern. M=85, Td=85 hrs | 細胞寿命 | infinite |
| ｸﾗｲｼｽＰＤＬ | 形態 | Cluster formation. | |
| 一般性状 | Derived from small cell lung carcinoma, classic type. Cells are prepared from heterotransplantation of the tumor into athymic BALB/c mice. Dense cored granule (neurosecretory granule) observed unde EM. | 細胞分類 | tumor |
| 細胞樹立者名 | Terasaki,T. and Shimosato,Y. | 細胞寄託者 | Terasaki,T. |
| 分譲時制限 | コメント | ||
| 入手年 | 2001 | 培養培地 | Eagle's minimal essential medium supplemented with glutamine and 10% heat-inactivated fetal calf serum. |
| 継代方法 | Same volume of a fresh medium as the cell culture was added to the cell suspension and pipetting twice or three times. Take a portion of the diluted cell suspension is applied to further cell culture. | 継代時細胞数 | split ratio = 1/2. |
| 人種 | Japanse | 炭酸ガス濃度 | 5 % |
| 採取組織名 | lung | 組織型 |
| ウイルスDNA・RNA検出検査 (Detection of virus genome fragment by Real-time PCR) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| ウイルスDNA 検出検査 |
tested | ウイルスRNA 検出検査 |
not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:2168867 | Selection of radioresistant cells by vitamin A deficiency in a small cell lung cancer cell line. Terasaki T,Shimosato Y,Wada M,Yokota J,Terada M Jpn J Cancer Res. 1990 Aug;81(8):780-5 |
| Pubmed id:2438038 | Reversible squamous cell characteristics induced by vitamin A deficiency in a small cell lung cancer cell line. Terasaki T,Shimosato Y,Nakajima T,Tsumuraya M,Ichinose H,Nagatsu T,Kato K Cancer Res. 1987 Jul 1;47(13):3533-7 |
| Pubmed id:6098504 | Interconversion of biological characteristics of small cell lung cancer depending on culture conditions. Terasaki T,Kameya T,Nakajima T,Tsumuraya M,Shimosato Y,Kato K,Ichinose H,Nagatsu T,Hasegawa T Gan. 1984 Dec;75(12):1089-99 |
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LOT Information
生細胞率、増殖速度、細胞濃度はバンクでのロット作製時の実測値として記していますが、保証値ではございません。また、倍加時間は継代間で計測したラフな値です。Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
| 細胞番号 | JCRB0169 | 細胞名 | |
|---|---|---|---|
| 培養ロット番号 | 06022003 | 培養種別 | distribution |
| 培地 | RPMI1640 medium with 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F) | 培養温度 | 37 C |
| 継代時細胞数（濃度） | uncountable due to aggregates. Split ratio 1:6-1:3 | 継代方法 | Dilution with pipetting large cell aggregates to small fragments. |
| 増殖速度 | 凍結時生細胞濃度 | 5 - 10 x 10^6 | |
| 凍結時生細胞率 | ca. 70 | 使用抗生物質 | free |
| 継代数 | > P31 | PDL数（プライマリ） | |
| ﾏｲｺﾌﾟﾗｽﾞﾏ検出 | - | 細菌汚染検出 | - |
| 真菌汚染検出 | - | ｱｲｿｻﾞｲﾑ検査・動物名 | Confirmed as human by NP, G6PD (type B), MD and LD. |
| 染色体モード | 染色体情報 | ||
| 表面抗原 | DNA Profile (STR) | ||
| 接着性 | No | 導入外部遺伝子 | |
| 凍結培地 | 10% DMSO, 20% FBS - RPMI1640 | 炭酸ガス濃度 | 5% |
| 解凍後生細胞率 | 追加情報 |
| 細胞番号 | JCRB0169 | 細胞名 | Lu-134-B |
|---|---|---|---|
| 培養ロット番号 | 04202026 | 培養種別 | distribution |
| 培地 | RPMI1640(GIBCO Cat #11875-093) with 10% heat inactivated fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K) | 培養温度 | 37 C |
| 継代時細胞数（濃度） | Uncountable(aggregates) | 継代方法 | This cell line grow with forming cell aggregates (suspension culture). At passage, disparse the cell aggregate to small cell clumps by pipetting and dilute to fresh medium. |
| 増殖速度 | NT | 凍結時生細胞濃度 | 9.2x10^5 |
| 凍結時生細胞率 | 85 | 使用抗生物質 | free |
| 継代数 | p33* | PDL数（プライマリ） | NT |
| ﾏｲｺﾌﾟﾗｽﾞﾏ検出 | - | 細菌汚染検出 | - |
| 真菌汚染検出 | - | ｱｲｿｻﾞｲﾑ検査・動物名 | NT |
| 染色体モード | NT | 染色体情報 | NT |
| 表面抗原 | NT | DNA Profile (STR) | D5S818:11 D13S317:9 D7S820:11 D16S539:9 VWA:17 TH01:9 AM:X TPOX:11 CSF1PO:12 |
| 接着性 | No | 導入外部遺伝子 | NT |
| 凍結培地 | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | 炭酸ガス濃度 | 5% |
| 解凍後生細胞率 | 65 | 追加情報 | Aggregate-forming cells; accurate cell counting is difficult. |
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