JCRB1952 LTD01
Cell information
Cell type：human iPS cells (View Pricing Information)
| JCRB No. | JCRB1952 | Cell Name | LTD01 |
|---|---|---|---|
| Profile | Healthy human peripheral blood mononuclear cell-derived iPS cell line (male). | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | |
| Identity | available | Tissue for Primary Cancer | |
| Case history | Healthy | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | ES-like | Character | Coat the dish surface with iMatrix-511 silk (nippi). |
| Classify | Established by | Hidenori AKUTSU | |
| Registered by | Hidenori AKUTSU | Regulation for Distribution | |
| Comment | Year | ||
| Medium | StemFit®︎ AK02N (Ajinomoto), Coat the dish surface with iMatrix-511 silk (nippi). | Methods for Passages | Passage when the cell density reaches 70-80%. Cells are collected by treatment of 50% TrypLE select in PBS with 0.5 mM EDTA. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | peripheral blood |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB1952 | Cell Name | LTD01 |
|---|---|---|---|
| LOT No. | 05282023 | Lot Specification | distribution |
| Medium | StemFit AK02N (Ajinomoto) | Temperature | 37 C |
| Cell Density at Seeding | 1-2x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with TrypLE™ Select CTS™ :0.5mM EDTA/PBS (-) =1:1, and cultured with iMatrix-511 (Matrixome) coated dishes,every 6-7days. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.16x10^6cells/0.5ml vial |
| Viability at cell freezing (%) | 84.5 | Antibiotics Used | free |
| Passage Number | p9 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,12 D13S317:8,12 D7S820:8,10 D16S539:10,12 VWA:17,18 TH01:6,9 AM:X,Y TPOX:8,11 CSF1PO:9,11 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | STEM-CELLBANKER GMP Grade (ZENOAQ) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 2x10^6 /vial | Additional information | Add 10uM Y-27632 when thawing and passaging cells. (remove next day) Start cell culture with four Φ60mm dishes. |