JCRB1558 RERF-LC-KJ/CMV-Luc
Cell information
Important Notice(s)発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines
Information for introduced genes, etc in luciferase expressing cell lines
Cell type：luciferase-expressing cancer cells (View Pricing Information)
| JCRB No. | JCRB1558 | Cell Name | RERF-LC-KJ/CMV-Luc |
|---|---|---|---|
| Profile | Luciferase stably expressing cell line (RERF-LC-KJ; Human lung cancer cell line). | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 78 year-old |
| Identity | available | Tissue for Primary Cancer | lung |
| Case history | lung cancer (adenocarcinoma, T4N3M1) | Metastasis | |
| Tissue Metastasized | Genetics | pLL3.7-luc transfected. Luciferase stably expressing cell line. Selection marker does not exist. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | |
| Classify | transformed | Established by | Murakami, T. |
| Registered by | Murakami, T. | Regulation for Distribution | To exchange and follow MTA between oversea end-user and cell-developer. |
| Comment | Year | 2011 | |
| Medium | RPMI1640 medium with 10% fetal bovine serum. | Methods for Passages | Harvest cells with 0.125% trypsin and 0.01% EDTA at 37 C for 5 minutes. |
| Cell Number on Passage | Split ratio=1/4. | Race | |
| CO2 Conc. | 5% | Tissue Sampling | |
| Tissue Type | lung |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB1558 | Cell Name | RERF-LC-KJ/CMV-Luc |
|---|---|---|---|
| LOT No. | 11062013 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) | Temperature | 37 C |
| Cell Density at Seeding | 1.04-4.86x10^4cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.125%trypsin(GIBCO) and 0.01% EDTA at 37 C for 5 minutes. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.71x10^6 |
| Viability at cell freezing (%) | 98.2 | Antibiotics Used | |
| Passage Number | p13* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10 D13S317:10 D7S820:10,11 D16S539:11 VWA:17 TH01:7,9 AM:X,Y TPOX:11 CSF1PO:12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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