JCRB0003 C3H/10T1/2-clone 8

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0003 Cell Name   C3H/10T1/2-clone 8
Profile   fibroblast, contact inhibited, (C3H mouse) Other Name   
Animal   mouse Strain   C3H
Genus   Mus Species   musculus
Sex    Age   
Identity    Tissue for Primary Cancer   embryo
Case history    Metastasis   No
Tissue Metastasized    Genetics   embryo
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   contact sensitive cell line
Classify   normal Established by   Reznikoff,C. et al.
Registered by   Koga,N. Regulation for Distribution   No regulation
Comment    Year   1985
Medium   Basal medium Eagle with 10% fetal calf serum Methods for Passages   0.02% EDTA and 0.25% trypsin. Subculture every 10 days. Avoid being confluent.
Cell Number on Passage   5x10^4 cells/60mm dish Race   
CO2 Conc.   5 % Tissue Sampling   embryo
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0190
Reference
Pubmed id:1277196In vitro correlates of transformation in C3H/10T1/2 clone 8 mouse cells.
Jones PA,Laug WE,Gardner A,Nye CA,Fink LM,Benedict WF
Cancer Res. 1976 Aug;36(8):2863-7
Pubmed id:129280Oncogenic transformation of C3H/10T1/2 clone 8 mouse embryo cells by halogenated pyrimidine nucleosides.
Jones PA,Benedict WF,Baker MS,Mondal S,Rapp U,Heidelberger C
Cancer Res. 1976 Jan;36(1):101-7
Pubmed id:1167940Repair of potentially lethal radiation damage in mammalian cells is associated with enhancement of malignant transformation.
Terzaghi M,Little JB
Nature. 1975 Feb 13;253(5492):548-9
Pubmed id:4812259Cell cycle dependency of oncogenic transformation induced by N-methyl-N'-nitro-N-nitrosoquanidine in culture.
Bertram JS,Heidelberger C
Cancer Res. 1974 Mar;34(3):526-37
Pubmed id:4796800Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division.
Reznikoff CA,Bertram JS,Brankow DW,Heidelberger C
Cancer Res. 1973 Dec;33(12):3239-49

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0003 Cell Name   C3H/10T1/2-clone 8
LOT No.   030195 Lot Specification   distribution
Medium   Basal Medium Eagle with 10% fetal bovine serum(JRS,D010400/JRHB,1B1062). Temperature   37 C
Cell Density at Seeding   2.5x10^3 cells/sq.cm Methods for Passages   Cells rinsed once with PBS(-) and treated with 0.25% trypsin & 0.02% EDTA for 3-8min at 37 C.
Doubling Time   NT Cell Number in Vial (cells/1ml)   8.3x10^5
Viability at cell freezing (%)   90.1 Antibiotics Used   free
Passage Number   P16 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LDH, G6PD, NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0003 Cell Name   C3H/10T1/2-clone 8
LOT No.   051589 Lot Specification   distribution
Medium   BME + FCS 10% Temperature   37
Cell Density at Seeding   5x10^4 cells/60mm dish Methods for Passages   0.02% EDTA and 0.25% trypsin. Subculture every 10 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P15 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0003 Cell Name   C3H/10T1/2-clone 8
LOT No.   021786 Lot Specification   distribution
Medium   Basal medium Eagle with 10% fetal calf serum. Temperature   37 C
Cell Density at Seeding   5x10^4 cells/ml Methods for Passages   0.02% EDTA and 0.25% trypsin. Subculture every 10 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3x10^6
Viability at cell freezing (%)   88% Antibiotics Used   free
Passage Number   P15 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0003 Cell Name   
LOT No.   110498 Lot Specification   distribution
Medium   Basal medium Eagle with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   3 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   ca 46 hrs Cell Number in Vial (cells/1ml)   6.7 x 10^5
Viability at cell freezing (%)   99.0 Antibiotics Used   free
Passage Number   P16 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0003 Cell Name   C3H/10T1/2-clone 8
LOT No.   09122013 Lot Specification   distribution
Medium   Basal medium Eagle with 10% fetal calf serum (FBS; GIBCO Cat. #10099). Temperature   37C
Cell Density at Seeding   approx. 3.4 x 10^4 cells/mL Methods for Passages   0.02% EDTA and 0.25% trypsin. Subculture every 10 days. Avoid being confluent.
Doubling Time    Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P16 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,NP,MD were examined. Mouse confirmed.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10 DMSO, 20% FBS - BME CO2 Conc.   5%
Viability immediately after thawing (%)   90.1% Additional information   
Cell No.   JCRB0003 Cell Name   C3H/10T1/2-clone 8
LOT No.   05182026 Lot Specification   distribution
Medium    Basal medium Eagle with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   approx. 4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1 day. Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   88.9 Antibiotics Used   free
Passage Number   P16 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - BME CO2 Conc.   5%
Viability immediately after thawing (%)   79.8 Additional information   
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