AyuK16C12 Trim71-K1 Cell information
Cell type：mousehomozygous mutant ES cells
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JCRB No.
AyuK16C12
Cell Name
Trim71-K1
Profile
Homozygous mutant mouse embryonic stem cell. Deficient gene: Trim71
Other Name
K16C12
Animal
mouse
Strain
(C57BL/6 x 129S4/SvJae)F1
Genus
Mus
Species
musculus
Sex
Age
Identity
not done
Tissue for Primary Cancer
embryo
Case history
Metastasis
Tissue Metastasized
Genetics
Trim71, tripartite motif-containing 71
Life Span
infinite
Crisis PDL
Morphology
ES-like
Character
pCMT-SAhygpA-NP22
Classify
Homozygous mutant
Established by
Horie, K.
Registered by
Horie, K.
Regulation for Distribution
Comment
Year
Medium
Knockout DMEM medium with 20% FBS, NEAA, sodium pyruvate, 0.1mM 2-mercaptoethanol, 1000U/mL LIF on mitomycin C-treated MEF feeder cells.
Methods for Passages
0.125% trypsin+0.01% EDTA, 0.1% gelatin coated dish
Cell Number on Passage
Race
CO2 Conc.
5%
Tissue Sampling
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.
AyuK16C12
Cell Name
Trim71-K1
LOT No.
10032014
Lot Specification
distribution
Medium
Temperature
37 C
Cell Density at Seeding
Methods for Passages
Doubling Time
NT
Cell Number in Vial (cells/1ml)
Viability at cell freezing (%)
Antibiotics Used
free
Passage Number
PDL
NT
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NT
Chromosome Mode
NT
Chromosome Information
NT
Surface Antigen
NT
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
NT
Medium for Freezing
CO2 Conc.
5%
Viability immediately after thawing (%)
NT
Additional information
